Poplar defense against insects: genome analysis, full-length cDNA cloning, and transcriptome and protein analysis of the poplar Kunitz-type protease inhibitor family.
Identifieur interne : 003521 ( Main/Exploration ); précédent : 003520; suivant : 003522Poplar defense against insects: genome analysis, full-length cDNA cloning, and transcriptome and protein analysis of the poplar Kunitz-type protease inhibitor family.
Auteurs : Ryan N. Philippe [Canada] ; Steven G. Ralph ; Carsten Külheim ; Sharon I. Jancsik ; Jörg BohlmannSource :
- The New phytologist [ 1469-8137 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN complémentaire (MeSH), Analyse de profil d'expression de gènes (MeSH), Analyse sur microréseau (MeSH), Animaux (MeSH), Clonage moléculaire (MeSH), Duplication de gène (MeSH), Expression des gènes (MeSH), Famille multigénique (MeSH), Gènes de plante (MeSH), Génome végétal (MeSH), Inhibiteurs de protéases (métabolisme), Lepidoptera (MeSH), Maladies des plantes (génétique), Peptides (MeSH), Phylogenèse (MeSH), Populus (génétique), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéomique (MeSH), RT-PCR (MeSH), Étiquettes de séquences exprimées (MeSH).
- MESH :
- génétique : Maladies des plantes, Populus, Protéines végétales.
- métabolisme : Inhibiteurs de protéases, Protéines végétales.
- ADN complémentaire, Analyse de profil d'expression de gènes, Analyse sur microréseau, Animaux, Clonage moléculaire, Duplication de gène, Expression des gènes, Famille multigénique, Gènes de plante, Génome végétal, Lepidoptera, Peptides, Phylogenèse, Protéomique, RT-PCR, Étiquettes de séquences exprimées.
English descriptors
- KwdEn :
- Animals (MeSH), Cloning, Molecular (MeSH), DNA, Complementary (MeSH), Expressed Sequence Tags (MeSH), Gene Duplication (MeSH), Gene Expression (MeSH), Gene Expression Profiling (MeSH), Genes, Plant (MeSH), Genome, Plant (MeSH), Lepidoptera (MeSH), Microarray Analysis (MeSH), Multigene Family (MeSH), Peptides (MeSH), Phylogeny (MeSH), Plant Diseases (genetics), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (genetics), Protease Inhibitors (metabolism), Proteomics (MeSH), Reverse Transcriptase Polymerase Chain Reaction (MeSH).
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Plant Proteins, Protease Inhibitors.
- chemical : DNA, Complementary, Peptides.
- genetics : Plant Diseases, Populus.
- Animals, Cloning, Molecular, Expressed Sequence Tags, Gene Duplication, Gene Expression, Gene Expression Profiling, Genes, Plant, Genome, Plant, Lepidoptera, Microarray Analysis, Multigene Family, Phylogeny, Proteomics, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
*Kunitz protease inhibitors (KPIs) feature prominently in poplar defense responses against insects. The increasing availability of genomics resources enabled a comprehensive analysis of the poplar (p)KPI family. *Using genome analysis, expressed sequence tag (EST) mining and full-length (FL)cDNA cloning we established an inventory and phylogeny of pKPIs. Microarray and real-time PCR analyses were used to profile pKPI gene expression following real or simulated insect attack. Proteomics of insect midgut content was used to monitor stability of pKPI protein. *We identified 31 pKPIs in the genome and validated gene models by EST mining and cloning of 41 unique FLcDNAs. Genome organization of the pKPI family, with six poplar-specific subfamilies, suggests that tandem duplications have played a major role in its expansion. pKPIs are expressed throughout the plant and many are strongly induced by insect attack, although insect-specific signals seem initially to suppress the tree pKPI response. We found substantial peptide coverage for a potentially intact pKPI protein in insect midgut after eating poplar leaves. *These results highlight the complexity of an important defense gene family in poplar with regard to gene family size, differential constitutive and insect-induced gene expression, and resilience of at least one pKPI protein to digestion by herbivores.
DOI: 10.1111/j.1469-8137.2009.03028.x
PubMed: 19780988
Affiliations:
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Philippe</name><affiliation wicri:level="4"><nlm:affiliation>Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Ralph, Steven G" sort="Ralph, Steven G" uniqKey="Ralph S" first="Steven G" last="Ralph">Steven G. Ralph</name></author><author><name sortKey="Kulheim, Carsten" sort="Kulheim, Carsten" uniqKey="Kulheim C" first="Carsten" last="Külheim">Carsten Külheim</name></author><author><name sortKey="Jancsik, Sharon I" sort="Jancsik, Sharon I" uniqKey="Jancsik S" first="Sharon I" last="Jancsik">Sharon I. 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We found substantial peptide coverage for a potentially intact pKPI protein in insect midgut after eating poplar leaves. *These results highlight the complexity of an important defense gene family in poplar with regard to gene family size, differential constitutive and insect-induced gene expression, and resilience of at least one pKPI protein to digestion by herbivores.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19780988</PMID><DateCompleted><Year>2010</Year><Month>05</Month><Day>27</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>184</Volume><Issue>4</Issue><PubDate><Year>2009</Year><Month>Dec</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Poplar defense against insects: genome analysis, full-length cDNA cloning, and transcriptome and protein analysis of the poplar Kunitz-type protease inhibitor family.</ArticleTitle><Pagination><MedlinePgn>865-84</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1469-8137.2009.03028.x</ELocationID><Abstract><AbstractText>*Kunitz protease inhibitors (KPIs) feature prominently in poplar defense responses against insects. The increasing availability of genomics resources enabled a comprehensive analysis of the poplar (p)KPI family. *Using genome analysis, expressed sequence tag (EST) mining and full-length (FL)cDNA cloning we established an inventory and phylogeny of pKPIs. Microarray and real-time PCR analyses were used to profile pKPI gene expression following real or simulated insect attack. Proteomics of insect midgut content was used to monitor stability of pKPI protein. *We identified 31 pKPIs in the genome and validated gene models by EST mining and cloning of 41 unique FLcDNAs. Genome organization of the pKPI family, with six poplar-specific subfamilies, suggests that tandem duplications have played a major role in its expansion. pKPIs are expressed throughout the plant and many are strongly induced by insect attack, although insect-specific signals seem initially to suppress the tree pKPI response. We found substantial peptide coverage for a potentially intact pKPI protein in insect midgut after eating poplar leaves. *These results highlight the complexity of an important defense gene family in poplar with regard to gene family size, differential constitutive and insect-induced gene expression, and resilience of at least one pKPI protein to digestion by herbivores.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Philippe</LastName><ForeName>Ryan N</ForeName><Initials>RN</Initials><AffiliationInfo><Affiliation>Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>Steven G</ForeName><Initials>SG</Initials></Author><Author ValidYN="Y"><LastName>Külheim</LastName><ForeName>Carsten</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Jancsik</LastName><ForeName>Sharon 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PubStatus="pubmed"><Year>2009</Year><Month>9</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>5</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19780988</ArticleId><ArticleId IdType="pii">NPH3028</ArticleId><ArticleId IdType="doi">10.1111/j.1469-8137.2009.03028.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Bohlmann, Jorg" sort="Bohlmann, Jorg" uniqKey="Bohlmann J" first="Jörg" last="Bohlmann">Jörg Bohlmann</name><name sortKey="Jancsik, Sharon I" sort="Jancsik, Sharon I" uniqKey="Jancsik S" first="Sharon I" last="Jancsik">Sharon I. Jancsik</name><name sortKey="Kulheim, Carsten" sort="Kulheim, Carsten" uniqKey="Kulheim C" first="Carsten" last="Külheim">Carsten Külheim</name><name sortKey="Ralph, Steven G" sort="Ralph, Steven G" uniqKey="Ralph S" first="Steven G" last="Ralph">Steven G. Ralph</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Philippe, Ryan N" sort="Philippe, Ryan N" uniqKey="Philippe R" first="Ryan N" last="Philippe">Ryan N. Philippe</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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